http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113444700-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-1105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
filingDate | 2020-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113444700-B |
titleOfInvention | Acetylacetone lyase mutant capable of improving acetylacetone synthesis efficiency, nucleotide, expression vector, recombinant bacterium and application |
abstract | The invention discloses an acetylacetone lyase mutant for improving acetylacetone synthesis efficiency, nucleotide, an expression vector, a recombinant bacterium and application, and belongs to the technical field of genetic engineering. According to the invention, through a site-directed mutagenesis method, lysine at the 15 th site of the acetylacetone lyase is changed into glutamine, alanine at the 60 th site of the acetylacetone lyase is changed into aspartic acid, and after the acetylacetone lyase mutant is used for synthesizing acetylacetone, the yield of acetylacetone is improved to 112.9 mg/L. |
priorityDate | 2020-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 137.