| abstract |
The present invention discloses the present invention relates to the technical field of pesticide residue detection, in particular to a detection method for methyl isoflavon residues in vegetables and fruits, which is characterized by comprising the following steps: 1) sample preparation: extract vegetables according to GB/T 8855 Take the edible part of the fruit sample, disperse it evenly, chop it into pieces, put it into a food processor and pulverize it, and fully homogenize and mix it to prepare the sample to be tested. 2) Extraction: Weigh 5g of sample into a 50mL plastic centrifuge tube, add 10mL of acetonitrile and 2g of sodium chloride, cover the centrifuge tube, and shake vigorously for 1min After centrifugation for 5 min, pipette 6 mL of supernatant into a 15 mL plastic centrifuge tube containing 900 mg of magnesium sulfate and 150 mg of PSA for later use; 3) Measurement: centrifuge for 10 min, accurately pipette 2 mL of supernatant into a 10 mL colorimetric tube. |