| abstract |
The invention discloses a proteinase K liquid enzyme preparation for molecular biology and in vitro diagnosis, and belongs to the field of enzyme preparations. In the proteinase K liquid enzyme preparation provided by the present invention, the proteinase K concentration is 10mg/ml≤C≤60mg/ml, the buffer concentration is 10mM-200mM, the Ca 2+ concentration is 0.01-10mM, and the addition amount of the solid form protective agent It is 1%-200% of the protein content, the addition amount of the protective agent in liquid form is 10%-200% of the volume of the enzyme liquid, and the total addition amount of the preservative is 0.01%-0.5%. The invention realizes that the residual activity of the proteinase K solution after being kept at room temperature for 12 months or incubated at 42°C for 10 days is more than 90%, and the long-term storage is free from RNase, DNase and microbial contamination, which simplifies storage, transportation and use. At the same time, the problem of easy precipitation of proteins at high concentrations is solved. |