http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113403300-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113403300-B |
| titleOfInvention | A method of using MOF material to enrich circulating free nucleic acid in body fluid and its application |
| abstract | The present invention relates to the technical field of separation and enrichment of circulating free nucleic acids in body fluids, in particular to a method for enriching circulating free nucleic acids in body fluids using MOF materials, comprising the following steps: (1) processing body fluid samples so that protein-bound proteins in the body fluid samples The circulating free nucleic acid is released from the protein-nucleic acid complex; (2) the MOF material is added to the solution treated in step (1), and the circulating free nucleic acid is adsorbed therein; (3) the adsorbed in the step (2) The MOF material separation of circulating free nucleic acid; (4) use EDTA to destroy the MOF material that has adsorbed circulating free nucleic acid in step (3), and release the adsorbed nucleic acid; (5) carry out the nucleic acid released in step (4) Purify to obtain circulating free DNA and circulating free RNA. The method of the invention has high enrichment efficiency, low cost, wide application range and high compatibility. |
| priorityDate | 2020-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.