http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113403232-A

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filingDate 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61e7890fcb0b4adae0b49a3c0030c495
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publicationDate 2021-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113403232-A
titleOfInvention A kind of method of screening phenanthrene class polycyclic aromatic hydrocarbon degrading bacteria
abstract The invention provides a method for screening phenanthrene polycyclic aromatic hydrocarbon degrading bacteria, which mainly comprises taking oil-contaminated soil and culturing it in a LB liquid medium containing crystal violet, and then after diluting according to a concentration gradient, coating it on a liquid containing crystal violet. The LB plate was inverted and cultured, and the single colony with no color circle was picked and inoculated into a liquid LB test tube for culture, and the decolorization rate was calculated. Cycloaromatic hydrocarbon degrading bacteria. This method uses crystal violet, which is a common triphenylmethane dye. When the observed sample can change from purple to light purple and finally colorless, it shows that the bacteria can degrade crystal violet and has the ability to degrade polycyclic aromatic hydrocarbons. The bacteria obtained by the screening method can efficiently degrade crystal violet and can also degrade polycyclic aromatic hydrocarbons.
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