http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113402635-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-265 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-206 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C08B37-0075 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08B37-10 |
| filingDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113402635-B |
| titleOfInvention | Method for separating and purifying heparin by expanded bed countercurrent chromatography |
| abstract | The invention relates to the technical field of heparin separation and purification, in particular to a method for separating and purifying heparin by an expanded bed countercurrent chromatography; anion exchange resin is used as a solid adsorption medium, the solid adsorption medium is added into an expanded bed countercurrent chromatography separation column, and a heparin solution is loaded to the expanded bed countercurrent chromatography for adsorption, elution, alcohol precipitation, centrifugation and washing to obtain purified heparin. The invention adds the solid separation medium commonly used in the fixed bed column chromatography into the separation column of the countercurrent chromatography, and forms the state of the expansion and dispersion of the adsorption medium in the separation column based on the countercurrent chromatography fluid dynamics balance principle to construct the expanded bed countercurrent chromatography, which can be used as a novel chromatographic separation method for separating polar polysaccharide heparin drugs. The method has the advantages of high sample processing amount, remarkably improved utilization rate of the adsorption medium, and good application prospect in separation and purification of polar polysaccharide bioactive macromolecules. |
| priorityDate | 2021-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.