http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113388559-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y603-02003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1229 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-04001 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113388559-B |
| titleOfInvention | A kind of recombinant escherichia coli and its application for glutathione synthesis |
| abstract | The invention provides a recombinant Escherichia coli for glutathione synthesis and its application. SpyCatcher and SpyTag are respectively connected to the N-terminus of bifunctional glutathione synthetase GshF and the C-terminus of polyphosphokinase PPK. The two passed gene fragments were simultaneously integrated into the same plasmid vector, and transformed into recombinant Escherichia coli. In the present invention, after the obtained recombinant Escherichia coli is cultured at a high density and the conditions for inducing expression are optimized, the maximum expression of the multi-enzyme complex is obtained, and the cells of E. coli are collected for efficient catalysis of the whole cell or assembled multi-enzyme complex. Centrifugation or membrane separation, simple recovery of cells or multi-enzyme complexes can be reused many times. The process effectively saves the production cost of glutathione, improves the utilization efficiency of the catalytic system through efficient repeated utilization, is simple to operate, and has a good application prospect. |
| priorityDate | 2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1046.