http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113388559-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_33a62710796e131599043055d399597f |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y603-02003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1229 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-04001 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate | 2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b7c6029093a6d04db7e180f4186068f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8dfe7871732dc97b75ead31ade8068f |
publicationDate | 2021-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113388559-A |
titleOfInvention | A kind of recombinant Escherichia coli for glutathione synthesis and its application |
abstract | The present invention provides a recombinant Escherichia coli for glutathione synthesis and application thereof. The two gene fragments that have been passed are simultaneously integrated into the same plasmid vector and transformed to obtain recombinant Escherichia coli. In the present invention, after high-density culture of the obtained recombinant Escherichia coli and optimization of inducing expression conditions, the maximum expression of the multienzyme complex is obtained, the Escherichia coli cells are collected for efficient catalysis of the whole cell or the assembled multienzyme complex, and the reaction is completed directly. Centrifugation or membrane separation, simple recovery of cells or multi-enzyme complexes can be reused many times. The process effectively saves the production cost of glutathione, improves the utilization efficiency of the catalytic system through efficient reuse, is simple to operate, and has good application prospects. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114774450-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114836447-A |
priorityDate | 2020-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1050.