| abstract |
The invention provides a recombinant protein, a recombinant expression vector, a recombinant cell, an NK cell activation magnetic bead, a preparation method and an application thereof, and relates to the technical field of NK cell activation propagation. The invention fuses the single-chain antibody and the Streptag2 label, has simple structure, ensures that the antibody expressed by the recombinant expression vector or the recombinant cell can be directly assembled with the magnetic bead without biotinylation modification, and the assembled magnetic bead can be directly used as an amplification stimulator of the NK cell, thereby effectively amplifying the NK cell. The NK cell activating magnetic bead has the binding capacity of more than 97 percent and high specificity; the killing efficiency of the NK cell line obtained by activating and culturing the NK cell activated magnetic beads is not obviously different from that of the NK cell activated and cultured by a feeder cell method. |