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filingDate 2021-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5a8fd3edd1971d93d0963421d832544
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publicationDate 2021-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113376282-A
titleOfInvention A kind of kit and detection method for lung cancer protein detection
abstract The invention discloses a kit and a detection method suitable for lung cancer protein detection. The detection method comprises mixing a heavy label peptide and a peptide segment after enzymatic hydrolysis of tumor tissue in a certain proportion, and then performing chromatographic separation and mass spectrometry scanning collection. Mass spectrometry data; wherein, in the mass spectrometry scanning acquisition process, after the DIA scanning mode is used, the PRM scanning acquisition is performed in parallel; this method can perform high-sensitivity absolute quantitative analysis of the target protein, and at the same time obtain the entire tumor full-spectrum proteome qualitative and quantitative information. The method of the invention has the advantages of convenience, rapidity, high throughput, high specificity and high accuracy; while ensuring the number of proteins in the detection result, the quantitative reliability of the target protein with low abundance is effectively improved. The reagents contained in the kit of the invention are all peptide dry powders, free of volatile, toxic and harmful substances, and have high safety. The experimental results obtained by the method are true and reliable, and have strong repeatability.
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