http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113373260-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
filingDate | 2021-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113373260-B |
titleOfInvention | An InDel marker primer for identification of cotton leaf nectar traits and its application |
abstract | The invention discloses an InDel-marked primer for identifying traits of cotton leaf nectaries and an application thereof. The InDel-marked primer comprises primer NEC1-F and primer NEC1-R, and the nucleotide sequence of primer NEC1-F is as shown in SEQ ID NO. 2. The nucleotide sequence of primer NEC1-R is shown in SEQ ID NO.3. The present invention takes Asian cotton leafy nectary material as male parent and Asian cotton leafless nectary material as female parent, crosses to obtain F 1 , and self-crosses F 1 to obtain F 2 , and determines F 1 and F 2 of leaf nectary traits. The F 2 population was used as a sample, and the population genome mixed pool re-sequencing analysis determined the candidate interval associated with the leaf nectaries, and used the perl script to develop 36 pairs of InDel markers in the signal interval. After synthesis, they were screened by polyacrylamide gel electrophoresis. The results showed that one pair of InDel markers showed polymorphism in the leafy nectary material and the leafless nectary material, and the corresponding NEC1‑F/NEC1‑R primers showed tight linkage in the leafy nectary cotton material. Relationship. |
priorityDate | 2021-08-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.