| abstract |
The invention belongs to the technical field of biomedicine, in particular to a culture method and application of a type I interferon receptor gene knockout mouse spleen reticular fibroblast immortalized cell line, which solves the problem of the primary immortalization of mouse FRCs established in the prior art. Although the virus infection rate is high, the virus content in the supernatant after virus infection is very low, and the expression of key cytokines is not high. The culture method of fibrous immortalized cell line includes using the spleen of type I interferon receptor knockout mice, adding digestive juice to digest mouse spleen fragments; using magnetic bead sorting and enrichment and accurate sorting by flow cytometry Sorting method; finally, FRCs cells were infected with SV40 large T antigen, screened, cloned and cultured for more than 50 passages, and a stable cell line was obtained. The cell line of the invention has short separation and culture period, low cost and no magnetic bead enrichment process. |