| abstract |
The invention provides a novel coronavirus SARS-CoV-2 broad-spectrum polypeptide antigen, a specific neutralizing antibody and application thereof, belonging to the technical field of virus immune detection. A novel coronavirus SARS-CoV-2 broad-spectrum polypeptide antigen, whose amino acid sequence is shown in SEQ ID NO: 1, reacts with SARS-CoV-2 human positive serum, and can specifically bind to novel coronavirus antibodies. Based on the polypeptide sequence of the present invention, using PCR, prokaryotic expression and protein purification technologies, a triple SARS-CoV-2 broad-spectrum polypeptide tandem fusion protein was prepared, simulating the trimer mode of the SARS-CoV-2 S protein in the natural state, and the fusion As an antigen, immunizing mice with protein can produce anti-SARS-CoV-2-specific neutralizing antibodies, and the neutralizing antibodies will have good application prospects in SARS-CoV-2 anti-infection treatment, vaccine development and detection kit development . |