| abstract |
The invention belongs to the technical field of immunofluorescence detection, in particular to an immunofluorescence method for detection of IgM antibody in fever with thrombocytopenia syndrome virus infection and a detection kit thereof, and solves the problem that the detection method in the prior art has high requirements on experimental instruments and operation It is cumbersome, and after the amplification is completed, the products need to be identified by steps such as electrophoresis and development, which is not suitable for the requirements of primary hospitals and on-site rapid detection. The fever with thrombocytopenia syndrome virus infection IgM antibody detection kit consists of 12 holes. 2 μL of cell suspension was added to each well to spread the slides, and after the cell suspension in the wells volatilized, they were fixed with cold acetone; the patient serum was diluted with PBS and added to the wells of the antigen slides, and one well was set for each antigen slide. Blank control, set positive control and negative control at the same time. The invention has relatively high specificity and sensitivity for detecting the pathogen of fever with thrombocytopenia syndrome, high detection accuracy, high detection efficiency and low detection cost. |