| abstract |
The invention discloses an avian influenza virus H9 type AlphaLISA detection kit and a detection method thereof, wherein the kit comprises the following substances A1) -A3) which are packaged independently: A1) a biotin-labeled avian influenza virus H9 type envelope protein hemagglutinin monoclonal antibody; A2) the receptor microsphere is coupled with an avian influenza virus H9-type envelope protein hemagglutinin monoclonal antibody; A3) streptavidin coupled donor microspheres; the nucleotide sequence for coding the avian influenza virus H9 type envelope protein hemagglutinin is shown as SEQ ID NO. 2. The detection method disclosed by the invention is simple, short in detection time, capable of realizing the purpose of rapid detection, free of expensive detection instruments and washing, low in detection cost, high in detection sensitivity, strong in specificity, free of false positive detection results, higher in detection reliability and more promising in market prospect. |