| abstract |
The invention discloses a method for detecting prawn VPAHPND acute strains and subacute strains, and belongs to the technical field of pathogen detection. The invention designs primers aiming at VPAHPND virulence factors PirA and PirB, and utilizes the primers to carry out multiplex PCR amplification, and the primers have the characteristics of strong specificity and high sensitivity; the multiple PCR method constructed by the primers is simple to operate and convenient to implement, can realize accurate identification of the VPAHPND acute strains and subacute strains of the prawns, is beneficial to making technical guidance for identifying the AHPND in different prawn farms, and is convenient for timely and pertinently preventing, controlling and treating the AHPND. |