http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113337585-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ee9cd7a9b53fb6bc07c846e20fa5cee7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2f62cb983b0560c1be4b4f3a922a1b7e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate | 2021-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_226facc4d5032475ca90305336055f3c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_288a02920ef49aaca7bc91213d239baf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3256f985a992948bec4a6b1391c7eec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33510fcb0d863e444dfd7dd8522cf123 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89413f3813f508ad9574e6f9456d8af0 |
| publicationDate | 2021-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113337585-A |
| titleOfInvention | A kind of small RNA quantitative detection method |
| abstract | The invention provides a small RNA quantitative detection method for non-disease diagnosis and treatment purposes. The method obtains cDNA by reverse transcription of poly(A)-tailed small RNA, and then performs real-time quantitative PCR amplification and detection on the cDNA , obtain the expression situation of the target small RNA, the sequence composition of the reverse transcription primer for reverse transcription is: 5'-tag sequence-Oligo dT-complementary sequence-3', wherein, the complementary sequence and the target small RNA 3' The terminal bases are complementary, and the tag sequence has no stem-loop structure. The invention can improve the specificity, sensitivity and detection efficiency of small RNA quantitative detection, and can realize simultaneous quantitative detection of various target small RNAs. |
| priorityDate | 2021-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.