http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113337539-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 |
| filingDate | 2021-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e12e3e0eb587ba58303603caa827de5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cc1057ea6f1ff128611a0e6a4f65e35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fe719be7c47190183fc6ae1370089b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01c45ae73914188a03f40ce52489c0b8 |
| publicationDate | 2021-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113337539-A |
| titleOfInvention | Method suitable for accurate and efficient gene editing of upland cotton |
| abstract | The invention relates to the technical field of plant genetic engineering and discloses a method for accurately and efficiently editing a gene suitable for upland cotton, wherein the nucleotide sequence of a vector is shown as SEQ ID NO. 2. The method for accurately and efficiently editing the gene of upland cotton is characterized in that a pRGEB32-GhU6.7-NPT II vector containing a cotton endogenous promoter pGhU6-7 is transformed, a replicon element in the BeYDV geminivirus is taken as a vector, an sgRNA sequence is constructed in a replication ring, a vector BeYDV-Cas9 with a gene editing function in cotton is constructed, GhPLA is selected as a target gene to verify the application of the BeYDV-Cas9 in cotton, and designs 1 target, introduces the BeYDV-Cas9 gene editing system into the cotton genome by utilizing agrobacterium-mediated genetic transformation, sanger sequencing is carried out on the transgenic plants, the editing efficiency type of the invention in the allotetraploid cotton genome is detected, therefore, the problems that the traditional CRISPR-Cas9 system cannot help to carry out gene editing on the upland cotton and a feasible and effective editing tool is unavailable are effectively solved. |
| priorityDate | 2021-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.