http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113337524-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2595314bedcd798b4b5ca2c017192de7 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 |
| filingDate | 2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c565fa521a45dd459d577902793715c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc4fb2df3d2250b5599082ae7b1c1a25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ebe17898e32cf93e2e3e6ef76eb41b59 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1aad7cb6b2ed91ff470af2c0fb7e55f0 |
| publicationDate | 2021-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113337524-A |
| titleOfInvention | Cloning expression method of resistance HPPD gene |
| abstract | The method for cloning and expressing the resistance HPPD gene comprises the following steps: the method comprises the following steps: sampling surface soil and sewage sludge to obtain samples, weighing 2.0g of the samples, adding the samples into a 50 mM MSM culture medium added with 2000 mu mol/L mesotrione, and carrying out enrichment culture at 30 ℃ and 180r/m for 5d to obtain a culture. The research separates and screens a mesotrione high-tolerance strain MST-5 from the soil polluted by herbicide, clones a mesotrione resistance gene Sthppd from the mesotrione high-tolerance strain MST-5, performs heterologous expression and purification on StHPPD by utilizing E.coli BL21(DE3), researches the resistance of the StHPPD to mesotrione, and aims to provide a basic material for the discovery and application of novel herbicide resistance genes. |
| priorityDate | 2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 81.