http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113324821-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1769d88f3c06ecbe6005121d948a043e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-84 |
| filingDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d50d5468e3128eb684dab9ab8b809499 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6824f0cdcff286edecbb36c3ed8523e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1bc10c95d25543a34507195fa8eeb7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59412f73cad526f5195efb67f6fe4231 |
| publicationDate | 2021-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113324821-A |
| titleOfInvention | A method to rapidly stain biological samples and obtain 3D data |
| abstract | The invention discloses a method for rapidly staining biological samples and obtaining three-dimensional data, comprising the following steps: S1, a first immersion step, S2, an embedding step, S3, a second immersion step, S4, a sample installation step, S5, The optical imaging step, S6, and slicing step, cycle and alternately perform S5 and S6 until the three-dimensional image of the biological sample tissue is obtained; wherein the imaging solution includes the following raw materials by mass percentage: 20-40% sugar or alcohol or sugar alcohol 10-30% urea; 0-30% penetration enhancer aqueous solution; the rest is dye. The invention uses an imaging solution composed of fructose, urea, a penetration enhancer and a dye to quickly replace the interstitial fluid inside the biological tissue sample, so that the newly exposed surface of the sample is quickly transparent and dyed, which greatly improves the dyeing efficiency. Microdissection and optical imaging can quickly acquire 3D data of stained biological samples of different volumes. |
| priorityDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.