http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113322303-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1b01718d1f98a48330dd078dbc16beb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate | 2021-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79990a809b12e6f29870fd523250acf0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc624d7855780e782c4ba50180cebc82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56b7ecd41d4c23a379c71c12f4721b82 |
| publicationDate | 2021-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113322303-A |
| titleOfInvention | Nucleic acid extracting solution for paraffin section sample and extracting method |
| abstract | The invention discloses a nucleic acid extracting solution for a paraffin section sample, which comprises the following components: 5 to 30 percent of liquid dewaxing agent, 10 to 300mM of Tris-HCl, 1 to 100mM of EDTA, 0.02 to 3 percent of LDS, 0.05 to 3M of guanidine hydrochloride, 0.05 to 5 percent of N-acetylcysteine, 1 to 30mg/ml of potassium chloride, 0.05 to 1M of sodium chloride, 0.02 to 2M of sodium hydroxide, 0.1 to 10 percent of triton and 0.1 to 5 percent of Tween 20. The nucleic acid extracting solution provided by the invention can provide a strong alkaline environment to promote the release of cell membrane broken nucleic acid, meanwhile, LDS can effectively remove impurities such as protein and the like, N-acetylcysteine can reduce the viscosity of a sample and assist the cell membrane breakage, the whole extraction process does not need complex operations such as dewaxing, lysate, washing, elution and the like, the complex operations such as a centrifuge, an extractor and the like are not needed, the operation is simple and convenient, the efficiency is high, and the extraction cost is reduced. |
| priorityDate | 2021-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.