| abstract |
The present invention relates to a preparation method of MTSase immobilized enzyme and MTHase immobilized enzyme and its application in trehalose production. The present invention is mediated by interacting polypeptide pair spyTag/spyCatcher, and the spyTag/spyCatcher can be used to specifically in vitro The characteristics of self-assembly to form covalently interacting short peptides, one-step recovery and immobilization of malto-oligosaccharide trehalose synthase MTSase and malto-oligosaccharide trehalose hydrolase MTHase, and double-enzyme transformation experiments to produce trehalose , through experiments, it was found that the immobilization carrier mediated by the interacting polypeptide to spyCatcher-CBM was used to immobilize the recombinase in the cell disrupting liquid, and it was found that the recovery rate of the recombinase through the immobilization reached 80.3%. The enzyme improves the temperature stability of the enzyme, the activity of the enzyme, and the reuse rate of the enzyme. |