http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113322222-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_752c595f9433837ceedeca04ae2a3310 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-555 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-555 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb09dc1f2b1eaba2139c4a485f47d922 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3489b4c035638feef31f01ad9f465842 |
| publicationDate | 2021-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113322222-A |
| titleOfInvention | Production process of genetic engineering bacteria for expressing feline omega interferon |
| abstract | The invention discloses a production process of a genetic engineering bacterium for expressing feline omega interferon, which comprises the following steps: the construction of the cat interferon engineering bacteria and the induction expression production process of the recombinant escherichia coli sequentially comprise fermentation, tank fermentation, centrifugation and crushing, inclusion body washing, denaturation, purification, renaturation and filtration sterilization. The process is simple and effective, and the cells of the negative control hole of the prepared genetically engineered bacteria do not produce anyThe obtained interferon has no toxic or side effect on cells; and a warp 10 4 Dilute cat omega interferon inhibits cytopathic effects by 100%, while 10 5 The diluted interferon had 3 wells with cytopathic effect, 10 6 Cytopathic effect of 5 wells of the double-diluted interferon was observed, and the specific activity of cat omega interferon against VSV was found to be 1.42X 10 7 U/mg。 |
| priorityDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.