abstract |
The invention belongs to the field of biotechnology, and discloses a method for producing malic acid, comprising the following steps: taking malic acid fermentation broth, centrifuging to remove sediment, collecting supernatant, then adding activated carbon, stirring, then adding diatomaceous earth, and stirring evenly , plate and frame filtration, collect the filtrate, and then perform microfiltration membrane filtration to collect the microfiltrate, pass through a chromatographic column filled with a strong base and weak acid type zwitterion exchange resin, so that the malic acid in it is adsorbed, and then eluted with hot water. The malic acid on the resin is repeatedly eluted, and the obtained eluates are combined, distilled under reduced pressure, and dried in vacuo to obtain the malic acid product. |