http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113308491-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 |
| filingDate | 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113308491-B |
| titleOfInvention | Recombinant plasmid and recombinant cell for co-expressing NFAT and human DNAM-1 protein, and construction method and application thereof |
| abstract | The invention provides a recombinant plasmid and a recombinant cell for co-expressing NFAT and human DNAM-1 protein, and a construction method and application thereof, and relates to the technical field of biological medicines. The invention provides a construction method of the recombinant plasmid, the recombinant plasmid obtained by construction, and a recombinant cell and a monoclonal recombinant cell constructed on the basis of the recombinant plasmid. The recombinant plasmid bidirectional promoter provided by the invention starts the expression of the DNAM-1 gene and the NFAT-Luciferase gene, and the gene expression is not influenced mutually. The recombinant cell is constructed based on the human immune type cell Jurkat, is more similar to an NK cell model, does not introduce viruses or cause cells to generate inflammation-related passages when the recombinant cell is constructed, and is simple to operate. The recombinant cell or the monoclonal recombinant cell can also be used for detecting and/or screening anti-human DNAM-1 antibodies. |
| priorityDate | 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 178.