| abstract |
The present invention relates to a recombinant coryneform bacterium with modified TK gene 5'-end sequence and its application. The recombinant coryneform bacterium of the present invention replaces the nucleotide sequence at positions ‑92 to ‑85 of the initiation codon TTG in the 5′ end sequence of the TK gene in the coryneform bacterium host, thereby reducing the secondary sequence of the 5′ end sequence of the TK gene The stability of the structure makes it easier to be transcribed or expressed. Compared with the original strain, the recombinant coryneform bacterium has a certain improvement in the L-lysine synthesis efficiency, thereby increasing the L-lysine yield, reducing the production cost, and compared with the existing high-yield L-lysine transformation method There is no conflict, but whether there is a mutual promotion with the existing transformation methods still needs to be further verified. |