http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113293201-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_89eeee2615888e854dc971e400b1a6f2 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
filingDate | 2021-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e407bcf22f9ea43564d0479be0d8e7f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_567e4cca1b31c776a8f8161b402dad5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd985b401785de8af9543877433f65f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8641de947602c73563b9c91b2b4ec963 |
publicationDate | 2021-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113293201-A |
titleOfInvention | Rapid quantitative detection method of novel chloramphenicol resistance gene and application thereof |
abstract | The invention relates to the technical field of environmental and biological detection, in particular to a rapid quantitative detection method of a novel chloramphenicol resistance gene and application thereof. The quantitative detection method comprises the following steps: (1) collecting and preprocessing an environmental sample; (2) extracting genome DNA; (3) sequencing the metagenome; (4) comparison of novel chloramphenicol resistance genes and statistics of the gene number of the 16S rRNA of the bacteria; (5) quantitative analysis of the novel chloramphenicol resistance gene. Compared with the prior art, the method is simple and easy to implement, does not need to construct standard plasmids, is not limited by sample types, has high detection timeliness and high comparison result accuracy, can accurately realize quantitative analysis of the gene in different environmental samples, greatly improves the timeliness of accurate detection, and is favorable for popularization in actual environmental sample monitoring. |
priorityDate | 2021-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Predicate | Subject |
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isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419533513 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID5959 |
Total number of triples: 16.