http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113293165-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-707 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1131 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2021-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113293165-B |
| titleOfInvention | A HEV-specific crRNA based on CRISPR-Cas12a technology, a detection kit and its application |
| abstract | The invention provides a HEV-specific crRNA based on CRISPR-Cas12a technology, a detection kit and application thereof, and belongs to the technical field of hepatitis E virus (HEV) detection. The present invention provides HEV-specific crRNA including four g RNAs based on CRISPR-Cas12a technology. Experiments show that the four gRNAs in crRNA can specifically target HEV of four specific genotypes, and have high binding specificity. The invention provides a detection kit for HEV comprising HEV-specific crRNA, CRISPR-Cas12a protease and a single-stranded DNA reporter system. The present invention combines isothermal amplification technology with CRISPR-Cas12a technology. The detection limit of this method can reach 10copies/μl, and the specificity is good, which provides an accurate, fast and simple detection method for clinical diagnosis and laboratory research. |
| priorityDate | 2021-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 54.