| abstract |
The invention discloses a hemostatic sponge and a preparation method thereof. The hemostatic sponge consists of three layers: the inner layer is a thrombin coating; the middle layer is prepared by crosslinking collagen, sodium alginate and antibacterial peptide by using a crosslinking agent glutamine transaminase, mixing with chitosan microspheres loaded with thrombin, and freeze-drying by two steps, wherein the outer layer is an electrostatic spinning layer consisting of polycaprolactone, chitosan, thrombin and tussah silk fibroin; wherein the mass ratio of the collagen, the sodium alginate, the chitosan microspheres containing thrombin and the antibacterial peptide in the middle layer is (30-40): (60-70): (30-50): (3-5); the mass ratio of polycaprolactone, chitosan, thrombin and tussah silk fibroin in the outer layer is (40-50): (30-40): (3-5): (10-30). Through the mode, the hemostatic sponge has good biocompatibility, antibacterial performance and hemostatic performance. |