http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113287524-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2021-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113287524-B |
| titleOfInvention | A method for detoxification of clover green vitrification by ultra-low temperature therapy |
| abstract | The invention discloses a method for detoxification by vitrification ultra-low temperature therapy of cloverleaf, belonging to the technical field of plant detoxification. The disclosed specific steps include: (1) pre-culturing the aseptic seedlings of cloverleaf; (2) cutting the shoot tips of the aseptic clover seedlings after the pre-cultivation in step (1), and performing differentiation culture to obtain cloverleaf (3) cutting the shoot tips of the three-leaf green seedlings obtained in step (2), firstly using the PVS 2 solution with a concentration of 50 to 60% to load the shoot tips, and then using the PVS 2 solution containing nano-calcium carbonate to load the shoot tips; The tip is dehydrated, and the container containing the PVS 2 solution and the shoot tip is frozen at an ultra-low temperature; (4) the shoot tip after the ultra-low temperature freezing in step (3) is thawed, and then recovered and cultured to obtain a cloverleaf virus-free seedling. The stem tips of Cloveria chinensis treated by the vitrification ultra-low temperature therapy detoxification method provided by the invention not only have a high survival rate, but also have a high detoxification rate of the surviving seedlings. |
| priorityDate | 2021-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.