| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be2c55a9de09e08e3404c0a5a1ae4dc7 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y604-01002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1288
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y603-04014
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-32
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-08007
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y602-01003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 |
| filingDate |
2021-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8de817753215ac62767ffdd1b922c70f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f45f0553471ad2debc26afda44cdf2f7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58398266bca2b6a41637eb29f4d0ce94 |
| publicationDate |
2021-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113278644-A |
| titleOfInvention |
Construction and fermentation method of a high-yielding artificial strain of Fengyuansu |
| abstract |
The invention provides a construction and fermentation method of a high-yielding strain of fenggenin; CRISPR gene editing is used to edit the 4'-phosphopantothel mercaptoethylaminotransferase gene sfp from Bacillus amyloliquefaciens FZB42 and the pleiotropic effects of Bacillus subtilis 168 The factor gene degQ and the strong promoter P43 were integrated into the genome of Bacillus subtilis 168 in tandem to construct the strain B.subtilis ds; the acetyl-CoA synthase gene acs of Escherichia coli BL21 and the acetyl-CoA carboxylase gene accACD of Salmonella enterica were used The biotin ligase gene birA of Corynebacterium glutamicum was connected in series to the expression vector pHT43, and then introduced into the plurigenin-synthesizing strain B.subtilis ds to construct a plurigenin high-yielding strain B.subtilis dspabacd; the constructed engineering strain was used for Shake flask fermentation culture to produce Feng Yuan element. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110713962-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110713962-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113969258-A |
| priorityDate |
2021-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |