http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113278525-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0668 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0667 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M23-04 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 |
filingDate | 2021-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113278525-B |
titleOfInvention | A kind of stem cell sphere or tumor sphere culture device and culture method |
abstract | The invention discloses a stem cell sphere or tumor sphere culture device, which comprises a cell culture plate and a microwell culture chip. The cell culture plate is composed of a bottom plate and an upper cover. The bottom plate is provided with a plurality of bottom plate holes, and the bottom of the bottom plate holes is closed; There are several growth holes on the well culture chip; the microwell culture chip is made of high molecular polymer material, such as PDMS, the thickness of the microwell culture chip is 0.5-7mm, and the diameter of the growth hole is 0.5-2.0mm; The well culture chip is located between the bottom plate and the upper cover. The invention also discloses a method for cultivating stem cell spheres or tumor spheres by using the device for culturing stem cell spheres or tumor spheres. The culture device and the culture method of the present invention are convenient for sample addition and liquid replacement, and can realize cell interaction research conveniently and quickly. Microwell Culture Chips are made of polymers for better hanging drop formation. The stem cell sphere or tumor sphere obtained by using the culture device of the present invention has a high survival rate and a good sphere forming rate. |
priorityDate | 2021-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.