| abstract |
The invention discloses a 3D printing biological ink based on gel microspheres and its application. The 3D printing bio-ink is formed from cross-linked cell-loaded gel microspheres, or obtained by mixing the cross-linked cell-loaded gel microspheres with one or more uncross-linked gel materials; The volume content of the gel microspheres of cells in the 3D printing bioink is 40% to 100%. The 3D printing bioink based on gel microspheres of the present invention has shear thinning and self-healing properties, and can directly 3D print low-concentration or low-viscosity gel materials without adding rheology modifiers, thereby realizing conventional 3D printing of gel materials at ultra-low concentrations, and endows difficult-to-print hydrogel materials with excellent printability; gel microspheres, as cell carriers, can provide a customized microenvironment for cell growth, proliferation, and differentiation, and can also Protecting cells from shear stress in the 3D printing process greatly promotes the application of 3D bioprinting technology in the construction of complex tissues/organs. |