http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113265477-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 |
| filingDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113265477-B |
| titleOfInvention | A method for detection of Salmonella typhimurium based on BCA-RPA and CRISPR-Cas12a system |
| abstract | The invention discloses a method for detecting Salmonella typhimurium based on BCA-RPA and CRISPR-Cas12a system, which is characterized by comprising the following steps: adding a nano-magnetic bead probe solution and an AuNPs probe solution to a test containing Salmonella typhimurium simultaneously In the solution, after shaking and reacting at room temperature for 45 minutes, magnetic separation and washing 3 times, and redissolving in enzyme-free water to obtain the MNB probe-bacteria-AuNPs probe sandwich structure, the MNB probe-bacteria-AuNPs probe The sandwich structure was subjected to RPA amplification reaction, and the reaction was carried out at 37 °C for 5 minutes. After the reaction, the fluorescence was observed under blue light for visual detection or quantitative detection with a microplate reader. The curve obtained according to the corresponding fluorescence intensity values of Salmonella typhimurium at different concentrations , the concentration value of Salmonella typhimurium in the solution to be tested is obtained, and the advantages are simple, rapid, high sensitivity and visual detection. |
| priorityDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.