| abstract |
The invention discloses a preparation method of hair follicle stem cells, comprising the following steps: step 1, after cleaning the dirt on the surface of the skin sample, sterilize and clean the skin sample; step 2, cut the skin sample into pieces, add neutral protease for digestion, Pull out the hair follicles and cut the hair follicle bulge; step 3, add trypsin for digestion, add stop solution to terminate the digestion; step 4, separate and collect the cells, transfer the obtained hair follicle stem cells to the hair follicle stem cell expansion medium to continue culturing ; Step 5, suck out the non-adherent cells together with the culture medium, and transfer the adherent cells to the serum-free medium for culture. By using the obtained skin samples to obtain hair follicle stem cells, after separation, expansion, purification, and culture, a large number of hair follicle stem cells can be obtained by cell proliferation to make up for the problem of insufficient hair follicle stem cells. |