http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113249407-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-072 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2207-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-203 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0278 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-7155 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2021-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113249407-B |
| titleOfInvention | Vector for homologous recombination and application thereof |
| abstract | The invention discloses a vector for homologous recombination and application thereof, wherein the vector for homologous recombination comprises: a homologous left arm, primer P2, an insert, primer P3, and a homologous right arm, wherein the primers P2 and P3 are capable of amplifying a sequence comprising a site of homologous recombination of a gene. The invention further provides a homologous recombination PCR identification method based on the homologous recombination vector. By using the method, no matter whether the target fragment is integrated to the correct position of the genome or not, the primer pair can amplify the PCR product, the result can be quickly judged, the occurrence of false negative condition is effectively reduced, the identification efficiency is improved, and the identification accuracy is improved. The identification of the mouse embryonic stem cell line prepared by homologous recombination and the knock-in mouse model shows that the vector has high-efficiency identification advantage and is suitable for establishing the homologous recombination cell line and the animal model. |
| priorityDate | 2021-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.