http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113249299-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_526173fcebb776be78f7b82ec64ceda4 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2015-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6a804c020d44846d7cc38d0077b29c9 |
| publicationDate | 2021-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113249299-A |
| titleOfInvention | Preparation method of double-layer skin containing microvascular lumen |
| abstract | The double-layer skin containing the microvascular lumen prepared by the invention has two layers of skin structures, namely a epidermis layer and a dermis layer containing the microvascular lumen from top to bottom; the epidermal layer is composed of epidermal cell germinal multiple layers, the dermal layer containing the microvascular lumen is constructed by fibroblasts, umbilical cord mesenchymal stem cells and umbilical vein endothelial cells, the fibroblasts, the umbilical cord mesenchymal stem cells and the umbilical vein endothelial cells are mixed according to a certain proportion, a multi-inoculation method is used, and the extracellular matrix secretion capacity and a paracrine system of the cells are utilized, so that on one hand, the histological structure of the skin dermis is formed, on the other hand, the proliferation, migration, lumen formation and stability of the endothelial cells are promoted. The tissue construction method simulates the assembly of normal human skin and achieves the formation of a dermis layer and the formation of a micro-lumen in the dermis through the interaction and the mutual influence among cells, thereby avoiding the instability of structure and function and the fussy operation caused by artificially adding an exogenous stent and a angiogenesis factor. |
| priorityDate | 2015-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 168.