| abstract |
The invention belongs to the field of bioengineering, and relates to a construction method of an LAMA3 gene knockout animal model based on a CRISPR/Cas9 technology, which comprises the steps of constructing sgRNA vectors of targeted mouse LAMA3 genes, transcribing and purifying the sgRNAs, obtaining and identifying F0 generation mice, obtaining and identifying F1 generation heterozygous mice, obtaining homozygous offspring and the like to construct the LAMA3 gene knockout animal model. |