http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113234745-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 |
| filingDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113234745-B |
| titleOfInvention | Method for realizing transmembrane transduction of marine microorganism low-temperature lipase gene by PTD-Tat |
| abstract | The invention is suitable for the technical field of fusion expression of transmembrane transduction gene fragments and lipase, and provides a method for realizing transmembrane transduction of a marine microorganism low-temperature lipase gene by using PTD-Tat, which comprises the following steps: 1. designing and obtaining a Lipase-tat fusion gene; 2. constructing pET28a-Lipase-tat vector: firstly, enzyme digestion Lipase-tat is carried out by applying Nco I and Xho I, and enzyme digestion fragments are recovered by glue; then, the pET28a vector is cut by enzyme with Nco I and Xho I, and a linearized pET28a vector fragment is obtained by gel recovery; then connecting the enzyme digestion fragment with the pET28a vector fragment to obtain a recombinant expression vector plasmid; 3. and (3) expressing and purifying and extracting the Lipase-tat protein pronucleus. Therefore, the invention can realize the transmembrane transduction of the marine microorganism low-temperature lipase gene, so that the weight-reducing medicine prepared by the recombinant protein can enter cells; meanwhile, theoretical and practical basis is provided for the transmembrane transduction mechanism of the PTD-Tat gene and the marine microorganism low-temperature lipase gene and the effective application of the PTD-Tat gene and the marine microorganism low-temperature lipase gene in the aspect of protein drug delivery. |
| priorityDate | 2021-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.