http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113234707-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-102 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-58 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2021-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113234707-B |
| titleOfInvention | A proteinase K mutant and its preparation method |
| abstract | The invention discloses a protease K mutant and a preparation method thereof, belonging to the technical fields of genetic engineering and enzyme engineering. The present invention provides a proteinase K mutant with improved thermostability, and optimizes the gene encoding the mutant. The mutant or gene is used to construct a recombinant expression vector and a recombinant expression cell, and the mutant that proteinase K activity and stability are all improved can be obtained without significantly reducing the expression amount, and the proteinase K mutant of the present invention is Under the condition of no protective agent, it still has more than 85% of the enzyme activity when stored at 25°C for 12 months, and the half-life at 60°C remains above 20 minutes, which is conducive to long-term stable storage and transportation. |
| priorityDate | 2021-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1032.