| abstract |
The invention relates to the technical field of stem cell culture, and discloses a human mesenchymal stem cell culture medium and application thereof. The medium of the present invention uses DMEM/F12 as the basic medium and adds the following components: human albumin, human transferrin, human insulin, fibronectin, cholesterol, sodium selenite, hydrocortisone, putrescine, β-Mercaptoethanol, Ethanolamine, L-Ascorbic Acid, bFGF, EGF, PDGF-BB, SCF, Niacinamide and Arcadisine. The invention provides a serum-free medium for mesenchymal stem cells. On the basis of optimizing the composition of the medium, four specific growth factors, acadisine and nicotinamide are added, which can enhance the proliferation ability of the cells, and can enhance the proliferation ability of the cells. During the process, it slows down the aging process of cells, and is suitable for primary culture of mesenchymal stem cells. |