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filingDate 2021-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08e99357a50bd7ff89590db928d6fc8a
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publicationDate 2021-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-113234180-A
titleOfInvention Method for preparing chondroitin sulfate from squid cartilage
abstract The invention discloses a method for preparing chondroitin sulfate by utilizing squid cartilage. Develops a green process and a new technology for extracting the chondroitin sulfate product by an enzymolysis method to obtain the squid chondroitin sulfate in different utilization ways. The method comprises the following steps: removing non-cartilage parts of squid cartilage, washing, crushing, adding 4% dilute alkali solution for degreasing, and repeatedly washing with distilled water to be neutral; adjusting pH to 2-3, performing enzymolysis for 24h, adjusting pH to neutral, adding sodium chloride to final concentration of 1.0mol/L, standing overnight, centrifuging for 30min, extracting supernatant, adjusting pH to 8.5-9, performing enzymolysis for 6h, heating, filtering, and repeatedly extracting filter residue once; precipitating with ethanol, redissolving the precipitate with distilled water, rotary evaporating, and freeze drying to obtain chondroitin sulfate product. The comprehensive extraction process adopted by the invention well keeps the natural structures and properties of collagen and chondroitin sulfate. Provides good technical support for better application of the composite material in preventing and treating diseases such as atherosclerosis, angina pectoris, hyperlipidemia and the like, and application of the composite material as biomedical engineering material in skin, bone and cartilage, nerve, heart and eye tissue engineering.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113429494-A
priorityDate 2021-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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