| abstract |
The invention discloses a method for determining the content of drugs and their metabolites in sewage, comprising the following steps: step 1, adjusting the pH value of a sewage sample to be less than 2, and filtering impurities for analysis; step 2, solid phase extraction: Before extraction, use methanol and ultrapure water to activate the solid phase extraction column in turn, mix the deuterated internal standard working solution and the sample to be tested, and then transfer to the solid phase extraction column, rinse with ultrapure water, and vacuum solid phase extraction. The column was eluted with 5% ammonia methanol solution, and the solid phase extraction column was vacuumed to collect the eluate; the eluate was blown to near dryness, 0.1% aqueous formic acid was added to mix well, and filtered with a 0.22 μm nylon microporous membrane as The test material sample extract is used for instrument detection; step 3, the test material sample extract is subjected to liquid chromatography-tandem mass spectrometry analysis to detect the content of each drug and its metabolites. The present invention basically includes all kinds of drugs involved in the existing cases, so as to meet the needs of practical work. |