| abstract |
The invention discloses an electrochemical sensor and its preparation method and application. The electrochemical sensor of the present invention includes, from bottom to top, activated screen-printed carbon electrodes, nano-gold particles and protein A, the protein A is modified with norovirus capsid protein monoclonal antibody, and the protein A on the Non-specific sites are blocked sites. The preparation process of the invention is as follows: after activation of the screen-printed carbon electrode, nano-gold particles, modified protein A, modified norovirus capsid protein monoclonal antibody are sequentially deposited, and non-specific sites are blocked to obtain an electrochemical sensor. When the electrochemical sensor of the present invention quantitatively detects the human norovirus capsid protein, the linear detection range is 1-10 ng/mL, and the detection limit is 0.635-1.0 ng/mL. The electrochemical immunosensor of the invention has the advantages of convenient operation, low cost and high sensitivity, and is suitable for rapid detection of norovirus on site. |