http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113219032-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-3271 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-327 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-48 |
| filingDate | 2021-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2023-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2023-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113219032-B |
| titleOfInvention | An electrochemical sensor for detecting hepatitis B exosomal miRNA and its preparation and application |
| abstract | The invention discloses an electrochemical sensor for detecting hepatitis B exosome miRNA and its preparation and application. The sensor includes a working electrode: the surface is immobilized with a capture probe sulfhydryl-labeled DNA nano-scaffold; the cascade chain displacement reaction (L-TEDCR) system induced by the target substance T chain: cascade T-type structure complex Ts, cascade reaction The auxiliary chain Fs and Ts are composed of DNA single-stranded P chain, R chain, L chain and L 0 chain, and the Fs is composed of DNA single-stranded F chain and L 0 chain; and MOF/DNA cascade enzyme amplification detection system. After the exosomal miRNA was added, L‑TEDCR was rapidly triggered to generate a large number of DNA nanoscaffolds; the generated DNA nanoscaffolds were immobilized on the electrode and combined with MOF/DNA cascade enzymes to generate a significant Amplified electrochemical signal. The sensor of the invention has the advantages of high sensitivity, fast detection speed, good specificity and the like. |
| priorityDate | 2021-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.