http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113215156-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8261 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-46 |
filingDate | 2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113215156-B |
titleOfInvention | A method for efficient creation of fragrant corn using CRISPR/Cas9 technology |
abstract | The invention discloses a method for efficiently creating fragrant corn by utilizing CRISPR/Cas9 technology. The invention designs targets for the exon regions of the maize ZmBADH2-1 gene and ZmBADH2-2 gene, constructs a CRISPR/Cas9 gene editing vector, and obtains a double-gene knockout mutant through genetic transformation. It was proved by experiments that in the grains of the four T1 double-gene knockout mutants, the highest 2-AP content was 438.29 μg·kg -1 and the lowest was 161.82 μg·kg -1 , while the unedited Jing 724 did not detect it. to 2‑AP. The invention utilizes the CRISPR/Cas9 technology to orientately and efficiently improve the aroma traits of maize from the molecular genetics level, and efficiently creates new germplasms of the maize backbone parents with different aroma gradients, which provides the development of aroma-type maize varieties and the improvement of the edible quality of maize. New breeding material. |
priorityDate | 2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.