| abstract |
The invention belongs to the technical field of molecular biology, and in particular relates to a method and application for extracting total microbial DNA from liquor Daqu or fermented grains. The technical problem solved by the present invention is to provide a method for improving the extraction quality of total microbial DNA by combining physical method, enzymatic method and kit method, aiming at the current situation of difficulty in DNA extraction from liquor Daqu or fermented grain microorganisms. In the method of the invention, the reagents are easy to obtain, and the operation method is relatively simple, and the method of combining enzymes and physics is used to break the walls, and the obtained genome DNA of fungi and bacteria is relatively complete, with high purity and stable extraction quality. The method of the invention is not only suitable for the extraction of the total DNA of Daqu microorganisms, but also has a good effect on the extraction of the total DNA of the wine grains. |