http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113213488-B
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C01P2004-04 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C01B33-12 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C01B33-12 |
filingDate | 2021-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-113213488-B |
titleOfInvention | Aptamer inverse opal photonic crystal sensing material and method for rapid and ultrasensitive label-free detection of Staphylococcus aureus enterotoxin B |
abstract | The invention belongs to the field of toxin detection, and relates to an aptamer inverse opal photonic crystal sensing material and a method for rapid, ultrasensitive and label-free detection of Staphylococcus aureus enterotoxin B. The aptamer inverse opal photonic crystal sensing material is prepared by a method comprising the following steps: S1. preparing polymethyl methacrylate microspheres; S2. preparing SiO 2 inverse opal photonic crystals; S3. preparing aptamer inverse opal Photonic crystal sensing material, S3‑1: amination modification; S3‑2: modification of colloidal gold on photonic crystal surface; S3‑3: adding SEB aptamer solution dropwise on the surface of inverse opal photonic crystal after modifying colloidal gold, The aptamer inverse opal photonic crystal sensing material is obtained. The present invention regulates the particle size when preparing PMMA microspheres, and optimizes the ratio of polymethylmethylpropionate microspheres to silica sol, buffer concentration and buffer type when detecting Staphylococcus aureus enterotoxin. The highly sensitive detection of Staphylococcus aureus enterotoxin can be realized under the optimal conditions. |
priorityDate | 2021-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.