http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113176361-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-324 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-32 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2021-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113176361-B |
| titleOfInvention | Identification method and application of bee pollen allergenic protein |
| abstract | The invention relates to the technical field of analysis and detection, and particularly relates to an identification method and application of bee pollen allergenic protein. The identification method of the sensitization protein in the bee pollen provided by the invention comprises the following steps: taking bee pollen freeze-dried powder as an identification sample, and performing protein extraction, protein enzyme digestion, peptide segment desalting and mass spectrum detection identification on the sample; in the protein extraction, a mixture of a sample and a protein extraction agent is subjected to a treatment means of intermittent ultrasonic disruption. The identification method provided by the invention solves the current situation that the identification of the bee pollen allergen is incomplete at present; meanwhile, the identification method of the invention obtains the amino acid sequence of the bee pollen potential allergen, analyzes the epitope recognized by T cells and B cells, and defines the sensitization attribute. The identification method provided by the invention is suitable for bee pollen of different plant sources, particularly 5 potential allergenic proteins in rape bee pollen are further determined by the identification method, and a basis is provided for subsequent quality safety control. |
| priorityDate | 2021-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 85.