http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113174442-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53b4d53983adbf56dd0e7d0bf96f8ce5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 |
| filingDate | 2021-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f0382a14520df73b613cd014eff6334 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2bc9d12717a1064a2a761b4536d654d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b14bdd800d9e532849d40634ba75745 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b553b2889ae4874498d0d543782e4a1c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3dd574e80ded9b611bf7828b9059d8a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_195420bbb3def51b34bf144b5db40e47 |
| publicationDate | 2021-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113174442-A |
| titleOfInvention | A quantitative detection method for the expression of key genes in the synthesis of tetramethylpyrazine in Daqu |
| abstract | The invention discloses a quantitative detection method for the expression of key genes in the synthesis of tetramethylpyrazine in Daqu, and belongs to the technical field of liquor detection. The invention adopts the real-time fluorescence quantitative method to detect the key genes (alsS gene and alsD gene) of tetramethylpyrazine synthesis in Daqu to detect the content change of tetramethylpyrazine. First, the genomic RNA in Daqu was extracted by the bacterial total RNA rapid extraction kit; then the RNA was converted into cDNA by the RevertAid First Strand cDNA Synthesis Kit (reverse transcription kit); and then real-time fluorescent PCR detection was performed to complete the tetramethyl Pyrazine content detection. The present invention utilizes molecular biology means to realize real-time quantitative detection of the alsS gene and alsD gene, which are key synthesis genes of tetramethylpyrazine, and evaluates the production of acetoin and tetramethylpyrazine by Bacillus. Compared with ordinary PCR methods, it has the advantages of simple operation, fast speed, and integration of product detection and quantitative analysis. |
| priorityDate | 2021-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 118.